Written in English
|Statement||by Janet Lynn Williams.|
|The Physical Object|
|Pagination||, 66 leaves, bound :|
|Number of Pages||66|
ties are also characteristic of the mllutanlt strains (le-scribed here. Materials and Methods Organisms. Six strains of C. reinhar(di were used in the experinments reported; wild type (strain c6 a mlutant strain of spontaneous origin, , an(d 4 UV-inducecl mutant strains, ac, ac, ac and(l ac (18). Wild type C. reinhardi is. The mutant, C-2A′, lacks the β, ϵ-carotenoids, α-carotene, lutein and loroxanthin. When grown under heterotrophic or mixotropic conditions this strain develops significantly higher levels of β-carotene and violaxanthin than does the original developmental mutant of Scenedesmus, C-2A′.The decrease in chlorophyll a and chlorophyll b observed in C-2A′ is accompanied by the near Cited by: In this study we present results obtained with the a-carotenoid-free mutant C-2AX of the unicellular green alga Scenedesmus obliquus, which is a sub-mutant of the greening mutant C-2A AlthoughX wx this mutant lacks the whole a-carotenoid-pool, i.e., lutein, a-carotene and loroxanthin, it is able to live autotrophically in the light THE unicellular green alga Scenedesmus obliquus (Chlorophyta), when grown photosynthetically, contains pigments (chlorophylls and carotenoids) similar to those of the chloroplasts of higher plants 1. Several mutant strains 1 have abnormal pigmentation 1. One of them, PG1, when grown in the dark accumulates the acyclic carotenoids phytoene (7,8,11,12,7',8',11', 12'-octahydro-ψ,ψ-carotene.
Heinze I, Pfündel E, Huhn M and Dau H () Assembly of light harvesting complexes II(LHC II) in the absence of lutein: A study on the alpha carotenoid free mutant C 2a′ 34 of the green alga Scenedesmus obliquus. Biochim Biophys Acta – Google Scholar. Abstract. Chlamydomonas reinhardtii double mutant npq2 lor1 lacks the β,ε-carotenoids lutein and loroxanthin as well as all β,β-epoxycarotenoids derived from zeaxanthin (e.g. violaxanthin and neoxanthin). Thus, the only carotenoids present in the thylakoid membranes of the npq2 lor1 cells are β-carotene and zeaxanthin. The effect of these mutations on the photochemical apparatus assembly. Chlamydomonas reinhardtii strains lacking phytoene synthase, the first enzyme of carotenoid biosynthesis, are white. They lack carotenoid pigments, have very low levels of chlorophyll, and can grow only heterotrophically in the dark. Our electron and fluorescence microscopic studies showed that such a mutant strain (lts) had a proliferated plastid envelope membrane but no stacks of. 2. Materials and methods Organisms, maintenance, growth and greening The wild type (WT) and mutant strains, WTLHC1 , WT-LHC3 and C-2A' , of Scenedesmus obliquus were grown either heterotrophically at 30 C as originally described  or autotrophically in a turbidostat as homocontinuous cultures .
responses exhibited by the Wt and Wt-lhc mutant of S. obliquus to UV-B irradiation with emphasis on the response of polyamines and carotenoids. Materials and methods. The functional and biochemical aspects of the photosynthetic apparatus in response to UV-B radiation were examined in unicellular oxygenic algae Scenedesmus obliquus. The wild type (Wt) and a chlorophyll b-less mutant (Wt-lhc) were used as a specific tool for the understanding of antenna role. Photosynthesis was monitored during and after UV-B stress by time resolved . AsDereillus giganteus (ref. 5) and Scenedesmus obliquus (ref. 6), and a geneiic study of the x n h (refs) will be discussed rater. Many chemicals also influence carote=nths!s." Most examples studied are inhibitory and the effects well known. In Ph om c 1 k 1 'n, several com ounds e.g. and compare four types of Scenedesmus obliquus pigment mutants with the wild-type strain. The specific nature of the carotenoids synthe-sized in both heterotrophically and myxotrophically-grown cells, Hill reaction activity, photoreduction activity, and total photosynthetic activity of these cells are examined. Mutant C-X-6E, lacking.